一、产品名称

中文名称：Anti-DNA-RNA杂交抗体(S96)(RGK60001)

二、产品描述

DNA-RNA杂交体是在真核细胞内普遍存在的一种自然现象，其在高转录活性位点的水平较高。这些非经典的核酸结构不仅具有转录调控功能，还可能导致基因座易发生染色体断裂。DNA:RNA杂交体的形成可以创建双链A/B中间构象，并在互补的被置换DNA链上提供第二个单链核酸结合蛋白的结合位点。研究表明，这些杂交体能有效抵抗DNA甲基转移酶的活性，其形成与多种神经系统疾病密切相关，例如，DNA:RNA解旋酶senataxin（SETX）的突变与显性青少年肌萎缩侧索硬化症4型及隐性眼动失协调2型的发生有关。

三、产品详情

• 名称：Anti-DNA-RNA杂交抗体(S96)(RGK60001)
• 货号：RGK60001
• 表达系统：哺乳动物细胞
• 种属反应性：一般
• 宿主：小鼠
• 同种型：IgG2a, kappa
• 克隆类型：单克隆
• 靶标：DNA-RNA杂交体
• 浓度：0.58 mg/ml
• 内毒素水平：请联系实验室获取相关信息
• 纯度：95%（由SDS-PAGE测定）
• 纯化方式：通过Protein A/G从细胞培养上清中纯化
• 克隆号：S96
• 应用：ChIP、ChIP-seq、DB、EMSA、FISH、ICC、IF、IP、SPR
• 状态：液体
• 保存溶液：0.01 M PBS, pH 7.4
• 稳定性和存储：使用手动解冻冷冻柜，避免反复冻融；短期存储于4°C（1-2周），长期存储于-20°C（12个月），或-80°C（长期）

四、参考文献

相关文献如下：

• Recognition of RNA by the S96 antibody creates pervasive artifacts when imaging RNA:DNA hybrids
• Immunoprecipitation of DNA:RNA Hybrids Using the S96 Antibody
• High-resolution, strand-specific R-loop mapping via S96-based DNA-RNA immunoprecipitation and high-throughput sequencing
• RNA/DNA Hybrid Interactome Identifies DXH9 as a Molecular Player in Transcriptional Termination and R-Loop-Associated DNA Damage
• The monoclonal S96 antibody exhibits highly variable binding affinities towards different R-loop sequences
• Structural basis of R-loop recognition by the S96 monoclonal antibody
• Best practices for the visualization, mapping, and manipulation of R-loops
• Catalytically inactive, purified RNase H1: A specific and sensitive probe for RNA-DNA hybrid imaging
• The Affinity of the S96 Antibody for Double-Stranded RNAs Impacts the Accurate Mapping of R-Loops in Fission Yeast
• The sub-nanomolar binding of DNA-RNA hybrids by the single-chain Fv fragment of antibody S96
• Modifications and interactions at the R-loop
• BRCA2 prevents R-loop accumulation and associates with TREX-2 mRNA export factor PCID2
• R-Loop Analysis by Dot-Blot
• Amplification-Free and Mix-and-Read Analysis of Multiplexed MicroRNAs on a Single Plasmonic Microbead
• New insight into the biology of R-loops
• Detection of Telomeric DNA:RNA Hybrids Using TeloDRIP-qPCR
• Ultra-deep Coverage Single-molecule R-loop Footprinting Reveals Principles of R-loop Formation
• Complexities due to single-stranded RNA during antibody detection of genomic RNA:DNA hybrids
• Development of electrochemical biosensors for simultaneous multiplex detection of microRNA for breast cancer screening

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